Scid mouse skin#
“After connecting the catheter tube with the cannula, the skin was closed with 6-0 silk sutures.” Kawakami, K, et al. “During the infusion period, the animals maintained their body weight as well as food and water consumption and showed no evidence of reduced motor function.” “The mice (athymic) were anesthetized by inhalation with 1.5% isoflurane.” Menon, J, et al. “Six- to 7-week-old nude mice were housed in a pathogen-free room in microisolators with autoclaved bedding and fed autoclaved rodent chow and water.” Song, P, et al. “Animals were anesthetized with 2.5% isoflurane during imaging (BLI).” Zhang, Y.M, et al. “Alzet pumps were aseptically filled with the specific solutions, and implanted subcutaneously into the left lateral flank (opposite to the tumor) of the mice under anesthesia (ketamine 100 mg/kg and xylazine 3 mg/kg), and the incision was closed by wound clips.” Dings, R.P.M, et al. Mice were “allowed to acclimate to local conditions for at least 1 week” P8817 Dings, R.P.M, et al. Temperature-controlled small water ‘blanket’ should be placed under the rodent during prolonged surgical procedures to prevent hypothermia. SCID mice, which virtually lack both T and B lymphocytes and accept xenogeneic cells, have been used during the last 5 years to provide a milieu for lymphocytes isolated from individuals with various autoimmune diseases, or for lymphocytes from mice that have a systemic lupus erythematosus-like syndrome.A cork board, plastic tray, or a few paper towels can be placed under the rodent to minimize heat transfer during short procedures.All injections, surgery, and pump preparation should be performed sterile in a laminar-airflow room or under a laminar-air-flow hood by sterile gowned, masked, and gloved operators and assistants.This usually results in the onset of one or more serious infections within the first few months of life. The defining characteristic is usually a severe defect in both the T- & B-lymphocyte systems. This reduces potential contamination by fur, feces, and bedding. SCID, Severe Combined Immunodeficiency, is a primary immune deficiency. The area should be subdivided into specific places for rodents awaiting or recovering from surgery, preparing rodents for surgery, and performing surgery.Rodent surgical area can be a room or part of a room that is easily sanitized and not used for other activities when rodent surgery is in progress.Establish special areas and equipment for use with immunodeficient mice only.Before surgery, lab bench or table should be cleaned and disinfected quaternary ammonium disinfectants or 70% alcohol are good choices.Biotechnology – Protein & Peptide Delivery.
Implantable infusion pumps for continuous administration of test agents to unrestrained laboratory animals.
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